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专利名称：Methods and compositions for DNA
manipulation
发明人：Jurate Bitinaite申请号：US13052536申请日：20110321公开号：US08440401B2公开日：20130514
摘要：single-stranded extension having a desired length and sequence composition.Methods for forming single-stranded extensions include: the use of a cassette containingat least one nicking site and at least one restriction site at a predetermined distance fromeach other and in a predetermined orientation; or primer-dependent amplification whichintroduces into a polynucleotide molecule, a modified nucleotide which is excised tocreate a nick using a nicking agent. The methods and compositions provided can be usedto manipulate a DNA sequence including introducing site specific mutations into apolynucleotide molecule and for cloning any polynucleotide molecule or set of joinedpolynucleotide molecules in a recipient molecule such as a vector of choice.
申请人：Jurate Bitinaite
地址：Rowley MA US
国籍：US
代理机构：New England Biolabs, Inc
代理人：Harriet M. Strimpel
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