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专利名称：DETECTION OF DIFFERENTIAL GENE
EXPRESSION
发明人：ROSENTHAL, ANDRE,HINZMANN,
BERND,SCHAEFER, REINHOLD,ZUBER,JOHANNES,TCHERNITSA, OLEG,GRIPS,MARTIN,HELLRIEGEL, MARTIN,SCHMITZ,ANNE-CHANTAL,SERS, CHRISTINE
申请号：EP01911558.3申请日：20010131公开号：EP1303531A2公开日：20030423
摘要：A nucleic acid (I) with differential expression between tumor and normal cellscomprising: (i) one of 550 fully defined sequences 250 nucleotides as given in thespecification; (ii) fragments of (i) with at least 50, preferably 200 nucleotides; (iii) asequence that hybridizes to (i) and/or (ii) under stringent conditions; or (iv) sequencescomplementary to (i)-(iii), is new. An independent claim is also included for testing theeffect of active compounds (A) on gene expression by treating a cell line with (A),isolating and labeling cellular RNA and hybridizing it to an array to prepare a geneexpression profile. - ACTIVITY : Cytostatic. - MECHANISM OF ACTION : Modulation of Rasactivity by inducing expression of tumor suppressor genes. Differential expressionanalysis between FE-8 cells (rat fibroblasts transformed with H-ras) and normal 208Ffibroblasts indicated differential expression of many hundreds of sequences. Treatmentof the transformed cells with the MEK-specific inhibitor PD98059 resulted in a normal
morphology and a significant reduction in capacity for anchorage-independentproliferation, despite a constant level of p21 ras. The inhibitor blocks the Raf/Meksignaling cascade and differential analysis of the treated cells showed that H-rasmediated down regulation of 36 transcripts was reversed and that upregulation of 25transcripts was blocked. The amounts of mRNA from 116 genes or expressed sequenceswere not altered by the inhibitor.
申请人：OLIGENE GMBH
地址：Jägerstrasse 51 10117 Berlin DE
国籍：DE
代理机构：Jungblut, Bernhard Jakob, Dr.
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